Chymotrypsinogen: 2.5-angstrom crystal structure, comparison with alpha-chymotrypsin, and implications for zymogen activation.
نویسندگان
چکیده
منابع مشابه
Calorimetric characterization of ligand binding on trypsinogen and chymotrypsinogen A, with evidence for two binding sites on alpha-chymotrypsin.
Heat is evolved when solutions of trypsinogen or chymotrypsinogen A are mixed with solutions of the amide or methyl ester derivatives of NQ-p-toluenesulfonyl-L-arginine, appropriate corrections being made for heats of reactant dilution. Exothermic reaction was also observed when the two ligands were mixed with tr-chymotrypsin; all measurements were made at 25”, pH 5.0. The dependence of apparen...
متن کاملZymogen activation in a reconstituted pancreatic acinar cell system.
Pathological activation of digestive zymogens within the pancreatic acinar cell initiates acute pancreatitis. Cytosolic events regulate this activation within intracellular compartments of unclear identity. In an in vivo model of acute pancreatitis, zymogen activation was detected in both zymogen granule-enriched and microsomal cellular fractions. To examine the mechanism of this activation in ...
متن کاملImplication of an ionizing group in the control of conformation and activity of chymotrypsin.
1. Acetylated chymotrypsinogen was prepared by the reaction of crystallized chymotrypsinogen with acetic anhydride at pH 6.7 and 4O, and purification of the product. End group analysis and Van Slyke amino nitrogen determination indicated that all the free Eand or-amino groups of the chymotrypsinogen molecule had been blocked by acetylation. 2. Activation of acetylated chymotrypsinogen by trypsi...
متن کاملCalorimetric Characterization of Ligand Binding on Trypsinogen and Chymotrypsinogen A, with Evidence for Two Binding Sites on &hymotrypsin*
Heat is evolved when solutions of trypsinogen or chymotrypsinogen A are mixed with solutions of the amide or methyl ester derivatives of NQ-p-toluenesulfonyl-L-arginine, appropriate corrections being made for heats of reactant dilution. Exothermic reaction was also observed when the two ligands were mixed with tr-chymotrypsin; all measurements were made at 25”, pH 5.0. The dependence of apparen...
متن کاملChymotrypsin C. I. Isolation of the Zymogen and the Active Enzyme: Preliminary Structure and Specificity Studies.
The chymotrypsins occur in pancreatic juice or extracts of fresh pancreas as inactive zymogens or chymotrypsinogens (1, 2). These chymotrypsinogens are converted to the various active forms by the action of trypsin and through autolysis. Two chymotrypsinogens, cationic chymotrypsinogen A (chymotrypsinogen a) (3) and anionic chymotrypsinogen B (4), have been isolated from bovine pancreas. Prelim...
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عنوان ژورنال:
- Biochemistry
دوره 9 9 شماره
صفحات -
تاریخ انتشار 1970